Structure and function of pif1 helicase
WebMar 10, 2024 · Pif1 Activity is Modulated by DNA Sequence and Structure. Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability. The Pif1 … WebAmong the enzymes thought to process these structures, the Pif1 DNA helicase is. G-rich sequences found at multiple sites throughout all genomes may form secondary structures called G-quadruplexes (G4), which act as roadblocks for molecular motors. Among the enzymes thought to process these structures, the Pif1 DNA helicase is
Structure and function of pif1 helicase
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WebAug 26, 2024 · Helicases are enzymes that convert the chemical energy stored in ATP into mechanical work, allowing them to move along and manipulate nucleic acids. The … WebJan 25, 2010 · 1. Introduction. Since the isolation of the Saccharomyces cerevisiae PIF1 gene more than 25 years ago [] and the eventual characterization of the S. cerevisiae Pif1 protein (ScPif1) as a helicase [], work on the Pif1 family of helicases has begun to elucidate the roles of these enzymes in both nuclear and mitochondrial genome stability.ScPif1 and …
WebOct 3, 2008 · Previous biochemical studies of human PIF1 were performed using N-terminal-truncated forms of PIF1, containing only the conserved helicase motifs, located in the C … WebNov 19, 2024 · In ensemble experiments, ATPase active full-length Pfh1 helicase from S. pombe has limited intrinsic dsDNA unwinding activity in the absence of a trap to prevent reannealing of the unwound strands (SI Appendix, Fig. S1).This behavior is similar to the one reported for Pif1 (45, 46) and is consistent with a rewinding activity counteracting …
WebNational Center for Biotechnology Information WebPif1 Activity is Modulated by DNA Sequence and Structure Biochemistry. 2024 Dec 21. doi: 10.1021/acs.biochem.1c00614. Online ahead of print. Authors David G Nickens 1 , …
WebAug 27, 2024 · Crystal structure of CaPif1 with bound DNA and ADP∙AlF 4. The 5′-tail of a dsDNA is required for Pif1 helicase to unwind the duplex. The Pif1 helicase core, CaPif1 (CaPif1 368−883), displayed a high affinity to poly(T), poly(C), poly(A), and G-rich ssDNA in the presence of an ATP analog (Fig. S1). Thus, CaPif1 was crystallized in complex ...
WebMay 8, 2013 · In vitro and in vivo, the yeast Pif1 helicase is able to unwind four-stranded G-quadruplex (G4) DNA efficiently and suppress the genomic instability that occurs at such … nad 50th anniversaryWebWhile they have an essential role in genome structure and expression, the rules dictating their processivity remain. Helicases are molecular engines which translocate along nucleic acids (NA) to unwind double-strands or remodel NA-protein complexes. While they have an essential role in genome structure and expression, the rules dictating their ... nad500 insulation boardWebJun 10, 2024 · Pif1 helicases, conserved in eukaryotes, are involved in maintaining genome stability in both the nucleus and mitochondria. Here, we report the crystal structure of a truncated Candida Albicans Pif1 (CaPif1 368-883 ) in complex with ssDNA and an ATP analog.Our results show that the Q-motif is responsible for identifying adenine bases, and … nad 501 cd playerWebDocument: The crystal structure of EAV nsp10" reveals a multi-domain organization of the arterivirus replicative helicase Because 3D structures of orthologous proteins were not available, we took advantage of the zinc-binding properties of nsp10 and used the zinc multiple-wavelength anomalous dispersion (MAD) method (42) to solve the EAV nsp10Ã … nad 50 yearsWebNov 1, 2008 · These results suggest that the functions of PIF1 might be restricted with particular situations and DNA structures. Issue Section: Nucleic Acid Enzymes … medicine for gerd coughWebAug 3, 2024 · The rescue of stalled DNA replication forks is essential for cell viability. Impeded but still intact forks can be rescued by atypical DNA helicases in a reaction known as fork regression. This reaction has been studied at the single-molecule level using the Escherichia coli DNA helicase RecG and, separately, using the eukaryotic SMARCAL1 … medicine for gerd prescriptionWebAug 26, 2024 · The Sf1 helicase core is comprised of four globular domains (1A, 2A, 1B and 2B), which together resemble a pair of tandem RecA-like folds with a single ATP-binding pocket residing in the center between domains 1A and 2A ( Figure 3 A) [ 35, 38, 45 ]. nad 40 days of prayer