Slowfade gold® antifade reagent invitrogen
WebbProLong Gold antifade mountant has become a widely used antifade reagent for our Invitrogen high-performance fluorescent dyes and stains because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial signal. WebbThe sections were then washed with TBS for 15 min, rinsed with distilled water, and mounted on glass slides using SlowFade Gold Antifade Mountant with DAPI (ThermoFisher Scientific, S36937). Sections were imaged on an automated epifluorescent microscope (Zeiss AxioImager Z1) using a 63× Plan-Apochromat 1.4 NA oil objective.
Slowfade gold® antifade reagent invitrogen
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WebbIt contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal. WebbAntifade Reagent ‡ SlowFade® Gold antifade reagent (Component G) 2 mL NA • ≤–20˚C • protect from light Reagents for Purifying Labeled Nucleic Acid Binding buff er (Component B) 6.0 mL NA • ≤25˚C Wash buff er (Component C) 3.2 mL NA Elution buff er (Component D) 6.0 mL NA Spin columns and collection tubes (Component E) 20 ...
Webb23 feb. 2024 · The slides were washed three times in PBTr, incubated with FITC-, TRITC-coupled secondary antibodies, or with Alexa-fluor 488/562 (donkey anti-rabbit), diluted in PBTr for 60–75 min at RT, washed three times with PBTr, then two times with PBS followed by mounting with SlowFade Gold antifade reagent with DAPI (Invitrogen, USA), sealed … Webb化工仪器网为您提供invitrogen 价格表【第15001条-第16000 ... s36937 slowfade gold antifade reagent 5 x 2 ml 1339.8. s36938 slowfade gold antifade reagent 10 ml 1293.6. s36939 slowfade gold antifade reagent 5 x 2 ml 1456. s37200 sulfate latex, 8% w/v 0.02 um 15 ml 1753.5.
WebbProlong および Slowfade 褪色防止用封入剤シリーズ Invitrogen™の褪色防止用封入剤は、どれもプレミックスタイプでそのまま使用可能です。 サンプルに1~2滴加えてマウントするだけです。 製品ごとに下記の特長を持ちます。 ご要望に合わせて最適な製品をご使用ください。 Invitrogenから初めてのライブセルイメージング用褪色防止剤 WebbElectrophoresis, Western Spotting both ELISA; Precast Electrophoresis Gels; Primary Antibodies
WebbComets were stained with SYBR Gold (S7563, Invitrogen) for 30 min at room temperature. Then, slides were rinsed in water and completely dried at 37°C. Last, slides were mounted with SlowFade Diamond Antifade Mountant (S36963, Invitrogen).
Webbfor SlowFade® Gold and ProLong® Gold reagents are available on our website at probes.invitrogen.com. Materials Kit Contents • SlowFade® antifade reagent (Component A), 10 mL in 50% glycerol (v/v) ready-to-use and sufficient for at least 200 coverslip-size … cta rechargeable batteryWebb9 dec. 2024 · Also, anti-V5 (R96125), Mito Tracker (M7512), goat serum (50062Z), ProLong Gold antifade reagent with DAPI (P36935) and ViraPower Lentiviral packing mix (K4975-00) from Invitrogen. Anti-ß-actin (A1978 ... and then mounted with Slowfade Light Antifade Kit (Invitrogen). All samples were measured by immunofluorescent microscopy in the ... c- tardWebbSlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence … earring card hangersWebbAbstract. Diabetic cardiac fibrosis increases ventricular stiffness and promotes diastolic dysfunction. The transient receptor potential ankyrin 1 (TRPA1) has been reported to be associated with the occurrence and development of various cardiovascular diseases. earring cards for jewelryWebb20 mars 2024 · Invitrogen™ SlowFade™ Gold Antifade Mountant. Liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. Brand: Invitrogen™ S36940. Code : NEW. Additional Details : Weight : 0.00680kg. View more … earring castings settingsWebb14 feb. 2024 · Tissues were washed three more times and stained with 1X DAPI (Cat# 62248; Thermo Fisher) in TBS for 10 min and mounted on microscope slides with cover slips and SlowFade TM Gold Antifade Mountant media (Cat# S36937; Invitrogen). cta recyclageWebbSlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can … earring chain