Hur antibody
Web25 However, although HuR acts as a positive regulator, CUGBP1 acts as a negative regulator of TJ/AJ proteins by competing with HuR for binding to the 3 0 UTR of occludin and E-cadherin. 22 In our ... Web2 dagen geleden · This article aims to develop a novel highly sensitive paper-based immunoassay. Here in, the paper zones were modified with GO and pyrene derivatives, the antibody immobilization strategy was illustrated in Scheme 1.Under optimal pH, Fc fragments of antibodies were pre-adsorbed onto GO/pyrene modified cellulose surface …
Hur antibody
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Web10 jan. 2024 · Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown...
Web14 mrt. 2024 · To determine the MUC16 mediated HuR target genes in TNBC, we performed RNA immunoprecipitation (RIP) with HuR antibody, followed by a tumor metastasis PCR array. First, HuR target genes were pull-down using HuR antibody from scramble control (HCC1806-SCR) cells and MUC16 knockdown (HCC1806-shMUC16) … WebIHC was performed as previously described. 29 After incubation with anti-Cleaved Caspase-3 or anti-HuR antibodies (#12,582) overnight at 4 °C, the samples were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 10 min at 37 °C. All imaged were obtained using an upright microscope (BX53, Olympus, Japan).
Web14 sep. 2024 · To this end, HuR HepKO mice were treated with an OPN-neutralizing antibody or control antibody while being fed the 60%-HFD-CD diet. OPN blockade resulted in a tendency toward reduced fibrosis in HuR HepKO mice compared to control antibody treatment, as shown by picrosirius staining (Figure 7D,E). WebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # MA1-167. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) and RNA Immunoprecipitation …
WebWe found that an mRNA-binding protein, human antigen R (HuR) associated with the 3′ UTR of AREG mRNA in response to UVB exposure, leading to enhanced mRNA stabilization. We also evaluated the …
WebHuR Antibody (3A2) is an IgG 1 κ mouse monoclonal HuR antibody (also designated ELAV like RNA binding protein 4 antibody or ELAVL4 antibody) suitable for the detection of the HuR protein of mouse, rat, human and Xenopus laevis origin by WB, IP, IF, IHC(P) and ELISA. HuR Antibody (3A2) is available as both the non-conjugated anti-HuR … kitchenaid crock pot slow cookerWebCompare anti-SERPINB13 Antibody ABIN1398419 from antibodies-online on Biocompare.com. Welcome Guest. Sign In Register. Products. Popular ... NCBI Gene Aliases headpin, HUR7, HU, 5430417G24, HURPIN, HSHUR7SEQ, HUR, PI13, PI, hea; Clonality Polyclonal; Research Area Proteolysis / Ubiquitin; Add to Compare List. Local … kitchenaid crushed ice attachmentWeb1 aug. 2011 · Anti-HuR antibody (Left) and anti-HuB antibody (Right) were used. U1 70 K was used as a loading control. β-tubulin III is a neuronal marker. ( E ) CoIP was carried out with nonspecific IgG or antibodies against hypophosphorylated RNAPII (8WG16), ser-2 phosphorylated RNAPII (H5), or ser-5 phosphorylated RNAPII (H14) using protein lysate … mabon wreathWebProduct Details Product Specific Information This antibody detects pS, pT and pY amino acids with very low cross reactivity to their non-phosphorylated variants Target Information There are three amino acids … mabo number 2WebAnti-HuR / ELAVL1 antibody [EPR17397] - BSA and Azide free (ab242410) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by recombinant technology for fast production Success from the first experiment – confirmed specificity through extensive validation maboo chudleighWeb1 dag geleden · The following primary antibodies were used for immunoblotting: antibodies against HA Tag (3724, CST), HuR (2582, CST), and pERK1/2 (4370, CST) were used at 1:1,000 dilution, and antibodies against Vinculin (13901, CST) and Tubulin (T6199, Sigma) were used at 1:3,000 dilution as the internal controls. kitchenaid crusty bread recipeWebAnti-HuR antibody was used in the IP ubiquitination assay. MG132-treated HCT116 cells were collected and incubated with Protein-A/G MagBeads (Yeason, Shanghai, China) and antibody. After overnight incubation, the immune complexes were centrifuged and washed, and the proteins were detected by Western blotting with an anti-ubiquitin antibody … kitchenaid crock pots