Fhbb protein

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August undefined, 2024

WebThe FH binding protein of T. denticola has been identified as FhbB and its atomic structure has been determined. The interaction of FH with T. denticola is unique in that FH bound to the cell surface is cleaved by the T. denticola protease, dentilisin. It has been postulated that FH cleavage by T. denticola leads to immune dysregulation in ... WebBackground: The Treponema denticola FhbB protein binds FH, a complement regulator.Results: The structure of FhbB was solved, and its interaction with FH was further defined.Conclusion: The structurally unique FhbB protein interacts with CCP7 of FH through electrostatic interactions.Significance: The T. denticola/FH interaction may …

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WebFeb 9, 2009 · FhbB is the smallest FH binding protein identified to date, and computer-based structural modeling suggests that it is unique in terms of its predicted structure . To identify FhbB determinants required for FH binding, truncation and random and site-directed mutagenesis analyses were conducted. WebNov 20, 2024 · FH bound to the cell surface receptor, FhbB (FH binding protein B), is competent to serve as a cofactor for the Factor I mediated-cleavage of the opsonin C3b. … blackstone bullnose worktop 3m 38mm/3mm

The Leptospiral General Secretory Protein D (GspD), a secretin, …

Webpallidum protein antigens expressed in Escherichia coli. Infect. Immun. 41:709-721. WebApr 17, 2024 · The factor H binding protein (FhbB) of the periodontal disease pathogen, Treponema denticola, served as a negative control antigen [45]. Statistical significance, was assessed using an unpaired, two-tailed student's t-test (95% CI, p < 0.05). All calculations were performed on GraphPad Prism 5 (GraphPad). WebThe factor H binding protein (FhbB) of the periodontal disease pathogen, Treponema denticola, served as a negative control antigen [45]. Statistical significance, was assessed using an unpaired ... blackstone built in counter

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Treponema denticola: FhbB, Dentilisin, Complement Evasion …

WebMar 2, 2024 · Inhibition of Plg-FhbB binding by ε-aminocaproic acid (a lysine analog) indicates that binding is mediated by electrostatic interactions that presumably occur with Lys binding sites contained within Plg “Kringle” domains 1, 2, 4 or 5. ... The data presented demonstrate that FhbB is a multi-functional protein that may contribute to ... WebThe Treponema denticola FhbB protein is a dominant early antigen that elicits FhbB variant specific antibodies that block factor H binding and cleavage by dentilisin. Infection and Immunity . PMID 27113359 DOI: 10.1128/Iai.01542-15 blackstone building omahaWebMar 2, 2024 · The T. denticolaFH receptor has been identified as FhbB, an 11.4kDa immunodominant lipoprotein. Three distinct subfamilies of FhbB proteins have been … blackstone built in cover

"WebThe Treponema denticola FhbB Protein Is a Dominant Early Antigen That Elicits FhbB Variant-Specific Antibodies That Block Factor H Binding and Cleavage by Dentilisin. Citing article. " - Fhbb protein

Fhbb protein

Identification of the primary mechanism of complement evasion b…

WebFactor H binding protein B (FhbB) is the sole FH binding protein produced by T. denticola. The interaction of FhbB with FH is unique in that FH is bound to the cell and then cleaved by the T ... WebJan 1, 2012 · Interestingly, the size of protein recovered by the pull-down assay was ∼50 kDa (as opposed to 150 kDa for FH). Based on its size and its detection by anti-FH antiserum it was originally concluded that this protein was FHL-1 and the T. denticola binding protein was designated as FhbB (FHL-1 binding protein B (McDowell et al. …

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WebApr 13, 2012 · To determine the structure of the T. denticola FhbB protein (GenBank TM accession number EF032156), recombinant protein was generated.The leader peptide (residues 1–23) was replaced with a 1.7-kDa hexahistidine tag and enterokinase cleavage site. The crystallization conditions have been previously reported (). The FhbB structure … WebThe data presented within demonstrate that FhbB is a multi-functional protein that may contribute to virulence through several mechanisms …

WebApr 13, 2012 · FhbB is the smallest (11.4 kDa) bacterially produced FH-binding protein identified to date ( 20, 21 ). FhbB binds and positions FH on the cell surface thus … WebThe gene encoding the Treponema denticola factor H-like protein 1 (FHL-1) binding protein, FhbB, was recovered and characterized. Sequence conservation, expression, …

WebThe Treponema denticola FhbB protein is a dominant early antigen that elicits FhbB variant-specific antibodies that block factor H binding and cleavage by dentilisin ... Infection and Immunity 84 (7), 2051-2058, 2016. 19: 2016: A di-iron protein recruited as an Fe [II] and oxygen sensor for bacterial chemotaxis functions by stabilizing an iron ... WebApr 1, 2024 · Recombinant Treponema denticola Factor H binding protein B (FhbB), generated as part of an earlier study, served as control in several experiments [26]. Recombinant proteins that partitioned in the insoluble fraction (GspD and GspD sub-fragments F1 through F5) were purified using urea-denaturing conditions and then …

WebJun 23, 2016 · The FhbB protein nomenclature used here is as follows: FhbB (FH binding protein B) is followed by a number designation to indicate phyletic type (FhbB1, FhbB2, …

WebThe Treponema denticola FhbB Protein Is a Dominant Early Antigen That Elicits FhbB Variant-Specific Antibodies That Block Factor H Binding and Cleavage by Dentilisin. Infection and immunity 2016-07 Journal article DOI: 10.1128/iai.01542-15 PMID: 27113359 PMC: PMC4936362 ... blackstone burner not lightingWebJul 1, 2011 · The gene that encodes T. denticola binding protein, FhbB, was found and characterized by McDowell et al. The characterization of this protein will be vital in the … blackstone built in gas griddleWebFhbB protein of the periodontal pathogen, T. denticola, and further define the molecular basis of its interaction with FH. FhbB possesses a previously undescribed protein fold … blackstone bungalows for saleWebJul 1, 1994 · The Treponema denticola FhbB protein is a dominant early antigen that elicits FhbB variant-specific antibodies that block factor H binding and cleavage by dentilisin. Infection and Immunity 2016 Journal article DOI: 10.1128/IAI.01542-15 EID: 2-s2.0-84976869378. Contributors ... blackstone business developmentWebApr 13, 2012 · FhbB possesses a unique fold that imparts high thermostability. The kinetics of the FH/FhbB interaction were assessed using surface plasmon resonance. A K (D) … blackstone burger recipesWebJun 11, 2024 · We previously demonstrated that Td binds the complement regulatory protein, factor H (FH), via the FhbB protein. FhbB is the smallest known bacterially encoded FH binding protein and its interaction with FH is unique. Full length FH is bound by FhbB at the cell surface and then FH is cleaved by the Td protease, dentilisin. A 50 kDa … blackstone burger press and sear kitWebOct 5, 2024 · Treponema denticola, a periopathogen, evades complement-mediated killing by binding the negative complement regulatory protein factor H (FH) to its surface via the FhbB protein. Paradoxically, bound ... blackstone butter wheel