Dgtp triphosphohydrolase inhibitor
WebJan 23, 2007 · Disclaimer Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional … WebThe enzyme dGTPase (EC 3.1.5.1) catalyzes the reaction . dGTP + H 2 O deoxyguanosine + triphosphate. This enzyme belongs to the family of hydrolases, specifically those acting on triphosphoric monoester bonds. The systematic name is dGTP triphosphohydrolase.Other names in common use include deoxy-GTPase, deoxyguanosine 5-triphosphate …
Dgtp triphosphohydrolase inhibitor
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WebIn 2001, Kicska et al. developed immucillin H, now better known as forodesine, as a small molecule PNP inhibitor (PNP-I). 4 The intention of the authors was to kill lymphocytes by elevating intracellular dG and consequently dGTP levels and to thereby eliminate leukemic cells. Forodesine was subsequently tested in clinical trials and was found ... WebDeoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a triphosphohydrolase that has a preference for dGTP over other dNTPs, they hydrolase dGTP to form deoxyguanosine and triphosphate. Escherichia coli dGTPase (Dgt) has been shown to have a strong single-stranded DNA-binding (ssDNA) activity [PMID:25694425] which is …
WebSep 16, 2013 · The bacteriophage T7 encoded inhibitor (gp1.2) of E. coli dGTP triphosphohydrolase WebOct 31, 2024 · The dNTP triphosphohydrolase SAMHD1 is a regulator of cellular dNTP pools. Given its central role in nucleotide metabolism, SAMHD1 performs important …
WebSep 25, 1993 · The dgt gene of Escherichia coli encodes a deoxyguanosine triphosphate triphosphohydrolase (dGTPase) that hydrolyzes dGTP to deoxyguanosine and tripolyphosphate. The enzyme is highly specific for dGTP which is hydrolyzed with a Km of 2-5 microM. Nitrocellulose filter binding assays demonstrate that, … WebMay 7, 2024 · However, the mechanism behind dGTP selectivity is unclear. Here we present the free-, ligand (dGTP)- and inhibitor (GTP)-bound structures of hexameric Ec-dGTPase, including an X-ray free-electron laser structure of the free EcEc-dGTPase and SAMHD1 share similar active-site and HD motif architectures; however, Ec
WebJan 22, 2024 · dNTP triphosphohydrolase (TPH) belongs to the histidine/aspartate (HD) superfamily and catalyzes the hydrolysis of dNTPs into 2′-deoxyribonucleoside and inorganic triphosphate. TPHs are required for cellular dNTP homeostasis and DNA replication fidelity and are employed as a host defense mechanism. PA1124 from the pathogenic …
The dNTPase activity of SAMHD1 is stimulated by dGTP24,25. dGTP, but not the other dNTPs, is also required for the formation/maintenance of the SAMHD1 tetramer (Fig. 1). A detailed view of the dGTP-binding site provides an explanation for the stabilization of the SAMHD1c′ tetramer by dGTP. Two dGTP … See more The SAM domain and its amino-terminal region are not required for tetramer formation or the dNTPase activity of SAMHD1 (refs 27, 28). However, SAMHD1c (120–626) … See more We have co-crystallized SAMHD1c′ with its dGTP/dATP substrates and solved the structure by the molecular replacement method, using 3U1N … See more To determine the importance for tetramer formation of dGTP/dATP binding in the allosteric site, we tested several SAMHD1 mutants. These … See more The substrate-binding pocket in the active site is formed by amino acids in the major lobe (Fig. 5b). Extensive interactions between the substrate dGTP and surrounding amino … See more normal bmi for adult womenWebSep 25, 1988 · Both the binding of the substrate dGTP and of the inhibitor gene 1.2 protein induce conformational changes in dGTPase. The conformation of the enzyme in the … how to remove old wall anchorsWebing of the substrate dGTP and of the inhibitor gene 1.2 protein induce conformational changes in dGTPase. The conformation of the enzyme in the presence of ... triphosphate … normal bmi in elderlyhow to remove old wax ring from toilet flangeWebDeoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a triphosphohydrolase that has a preference for dGTP over other dNTPs, they hydrolase dGTP to form … normal bmi levels for womenWebDownload Table Data collection and refinement statistics for Dgt crystals with and without DNA from publication: Structure of Escherichia coli dGTP Triphosphohydrolase: A Hexameric Enzyme With ... normal bmi women 5\u00277WebApr 1, 1990 · The dgt gene lies immediately upstream of htrA and 6 kilobases from dapD, in the same region as the optA1 mutation. The dgt structural gene is 1515 base pairs, encoding a protein of 59,315 daltons, in agreement with the size and N-terminal amino acid sequence of the purified protein. An E. coli strain containing a null allele has no … normal bmi number for women