Ctd110.6 antibody

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August undefined, 2024

WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... WebSimilar results were obtained for wild-type HEK293T cells (not shown). (C) Expression and O-GlcNAcylation of Notch1, Notch1Δ2xO-GlcNAc and Notch1Δ4xO-GlcNAc were analyzed by immunoprecipitation (IP) using NOTCH1 (8G10) antibody, followed by immunoblotting with CTD110.6 O-GlcNAc or NOTCH1 antibodies.

O-GlcNAc Monoclonal Antibody (CTD110.6) (61453)

WebResults: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized. WebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at … philip hoffman obituary

Anti-O-GlcNAc (CTD110.6) antibody Monoclonal Antibodies

WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87]. WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation. WebAntibody: Clone: CTD110.6: Immunogen : This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b … philip hogg trainers

Phospho-VE-cadherin (Tyr731) Polyclonal Antibody

WebBinding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell surface … WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Crossreactivity: philip hogrefeWebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). philip hoffman twister

"WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit " - Ctd110.6 antibody

Ctd110.6 antibody

Characterization of a mouse monoclonal antibody specific for O …

WebFor O-glcNAcylation detection, CTD110.6 antibody was covalently conjugated to (4) Defective STIM-mediated store operated Ca2+ entry in hepatocytes leads to metabolic dysfunction in obesity eLife December 15, 2024 Ana Paula Arruda, Benedicte Mengel Pers, Günes Parlakgul, Ekin Güney, Ted Goh, ... WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 …

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WebJan 13, 2003 · Anti-CD46 antibodies inhibit binding of Ad7 and Ad11 virions to CHO-BC1 cells. In a second experiment, we preincubated A549 cells or CHO-BC1 cells with … http://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21

WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … WebWestern blotting of SDS-PAGE gels with O-GlcNAc-specific antibodies (RL2 and CTD110.6) shows that platelets contain 15-20 major O-GlcNAcylated proteins. Upon stimulation by agonists ( e.g. thrombin, TRAP, collagen, U46619, and A23187), the extent of modification displays protein- and agonist-specific changes. Treatment of platelets with …

WebFigure 6 HRECs exposed to TNF-alpha, and high glucose for 24 h demonstrated alterations in VE-Cadherin and defective complex formation. The whole cell lysates were immunoprecipitated with indicated antibodies, and the immunocomplexes were analyzed for O-GlcNAcylation using anti-O-GlcNAc antibody (clone CTD110.6) ( A ).

WebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ...

WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O-GlcNAc (CTD110.6) antibody Invented at ... truffels effectWeb丁香通商铺上海远慕生物科技有限公司为您提供细胞库 / 细胞培养、技术服务、试剂、原辅料包材、抗体、elisa 试剂盒、实验室仪器 / 设备、耗材等分类产品1720件，全方位满足您的科研实验采购需求 truffeltherapieWebO-GlcNAc monoclonal antibody (CTD110.6) (purified) O-GlcNAc单克隆抗体（CTD110.6）（纯化的） ... VEGF Recombinant monoclonal antibody (A.4.6.1) (Mouse IgG1κ) 血管内皮生长因子重组单克隆抗体（A.4.6.1）（小鼠IgG1κ） ... philip hogh rottenburgWebJul 15, 2014 · The antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought to accumulate in cells experiencing severe glucose deprivation. In this study, we have addressed two questions: (1) Which other antibodies … philip hog sneakers onlineWebO-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PLoS One. 2011 04 19;6(4):e18959. Zachara, Natasha E; Molina, Henrik; Wong, Ker Yi; Pandey, Akhilesh; Hart, Gerald W. The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA … philip hogg shoesWebO-GlcNAc Antibody (CTD110.6) is an IgM κ mouse monoclonal O-GlcNAc antibody (also designated O-linked β-N-acetylglucosamine antibody, O-β-GlcNAc antibody, O-linked … truffe mycorhizeWebSpecificity. Clone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The … philip hog sneakers