Ctcf antibody chip

WebDec 11, 2015 · Chromatin was obtained and subjected to fragmentation with an average length of 300 bp by sonication. The anti-PolII monoclonal antibody 8WG16 (Covance, MMS-126R) and anti-CTCF antibody (Abcam, ab70303) were used to enrich PolII and CTCF-bound chromatin fragments, respectively. ChIP DNA on beads was used for ChIA … WebInvitrogen Anti-CTCF Polyclonal, Catalog # PA5-17143. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP), ChIP assay (ChIP) and RNA Immunoprecipitation (RIP) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat samples. Supplied as 100 µL purified antibody (14 µg/mL).

Cut-C: cleavage under tethered nuclease for conformational …

WebFeb 12, 2024 · ChIP was performed with 2 µl of rabbit polyclonal antibody crude sera against CTCF 1–293 or Cp190 1–1096, each incubated with half of the chromatin prepared from a biological replicate ... WebCTCF antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with MCF7-SER breast cancer cell line chromatin and 4 µg of … cindy mariner https://modernelementshome.com

ChIPAb+ CTCF - ChIP Validated Antibody and Primer Set

WebThe ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG), and qPCR primers which amplify a 165 bp region of human H19 internal … WebJan 14, 2024 · As a complement to our genome-wide comparisons of CTCF and cohesin binding, we performed a thorough analysis of CTCF antibody performance in ChIP-seq, finding that a number of antibodies fail to detect the full spectrum of CTCF sites (SI Appendix, Fig. S3). This observation suggests that the high prevalence of CNC sites in … WebAug 23, 2024 · Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res. 2024;45:4606-4618 pubmed publisher Jian X, Felsenfeld G. Large parental differences in chromatin organization in pancreatic beta cell line explaining diabetes susceptibility effects. cindy markison licsw

CTCF Polyclonal Antibody (PA5-17143) - Thermo Fisher Scientific

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Ctcf antibody chip

Anti-CTCF Antibody serum, Upstate® Sigma-Aldrich

WebMar 25, 2011 · To confirm that the observed effects of HDAC inhibition were due to increased histone hyperacetylation at the promoter of Glut-4, we carried out chromatin immunoprecipitation (ChIP) analysis at the Glut-4 promoter in A549 cells treated with TSA. A no-antibody control was included to test for non-specific carriage of DNA with histones. WebCTCF antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa …

Ctcf antibody chip

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WebFor ChIP-seq, chromatin was extracted and immunoprecipitation with antibodies was performed. All IPs in one biologocal replicate samples were processed together. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina kit and using NEBNext Multiplex Oligos. Libraries were amplified with 6-8 cycles of PCR. WebCTCF Rabbit pAb (A12562) Western blot analysis of extracts of various cell lines, using CTCF antibody (A12562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Web11 rows · Applications. Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC … WebUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor. An …

WebChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9. AbFlex® CTCF antibody (rAb) tested by Western blot. 20 µg of HeLa … WebAnti-BPTF antibody produced in rabbit. ... (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. ... (ChIP), ChIP-seq and Western Blotting ...

WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408) Research with confidence – consistent and reproducible results with every batch. Long …

WebChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade – BSA and Azide free (ab240035) Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab128873. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 … diabetic cinnamonsugarsuggestionsWebMonoclonal Antibody for studying CTCF. Cited in 110 publications. Validated for WB, IP, IHC, IF, IF, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, CTCF (D31H2) XP® … diabetic classes in cincinnatiWebProduct Information. 10915-1-AP targets CTCF in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. … diabetic classes in tucsonWebJul 29, 2024 · Antibody incubation was performed for 2 h with the 0.02% digitonin-containing antibody buffer, which contained either 1 μg anti-H3K4me3 (Abcam; ab8580) or 1 μg anti-CTCF antibody (Abcam; ab70303) as described above. Cells were washed and resuspended with 0.02% digitonin/ wash buffer by centrifugation at 500×g for 5 min at 4 … diabetic classes san diego countyWebChromatin Immunoprecipitation: An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells. Use Anti-CTCF … diabetic classes johnstown conemaugh hospitalWebMar 1, 2024 · Figure Legend Snippet: ChIP-qPCR assay was conducted to identify epigenetic factors nearby EBV CTCF binding sites between B95-8 and SNU719 cells; A) enrichment of CTCF at the suggested CTCF binding sites, B) enrichments of RNAP II at the suggested CTCF binding sites, C, D) enrichments of histones such as H3K4me3 and … diabetic chutney with pomegranateWebSep 4, 2024 · Except the ChIP-seq data for the above explored factors, there is still a large amount of untapped ChIP-seq data (), which might contain new co-localized CTCF co-factors.However, a desirable prerequisite for the analysis of co-factor co-localization is that the ChIP-seq experiments should be conducted in the same cell type under the same … cindy marlenee